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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
21/05/2014 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BUSTAMANTE, F. O.; ROCHA, L. C.; TORRES, G. A.; DAVIDE, L. C.; MITTELMANN, A.; TECHIO, V. H. |
Afiliação: |
FERNANDA O. BUSTAMANTE, UFLA; LAIANE C. ROCHA, UFLA; GIOVANA A. TORRES, UFLA; LISETE C. DAVIDE, UFLA; ANDREA MITTELMANN, CNPGL; VÂNIA H. TECHIO, UFLA. |
Título: |
Distribution of rDNA in Diploid and Polyploid Lolium multiflorum Lam. and Fragile Sites in 45S rDNA Regions. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Crop Science, v. 54, n. 2, p. 617-625 2014. |
DOI: |
https://doi.org/10.2135/cropsci2013.05.0325 |
Idioma: |
Inglês |
Conteúdo: |
The distribution, number, and location of ribosome DNA (rDNA) regions on chromosomes were evaluated, and transcriptional activity was described in genotypes of Italian ryegrass (Lolium multiflorum Lam.), as well as in an offspring resulting from interbreeding. Nucleoli and nucleolar organizer regions were labeled with silver nitrate and fluorescent in situ hybridization (FISH) was performed with 5S and 45S rDNA as probes. Hybridization procedures were performed on slides previously stained with the Ag-NOR method. The 5S rDNA sites were highly conserved, while 45S rDNA sites had wide variability, even showing more than one site on the same chromosome. One of the genotypes had more than expected 45S rDNA sites. Approximately 95% of the cells at metaphases had at least one chromosome break/gap in the 45S rDNA site, resulting in chromosome fragmentation. Thus, 45S rDNA correspond to fragile sites in L. multiflorum. These events can affect genome organization and cause new chromosomal rearrangements which, along with some other factors, might be responsible for microevolutionary changes involved in differentiation and speciation. Not all 45S rDNA sites are transcriptionally active. Variation in both the number and size of nucleoli and mechanisms of nucleolar fusion were observed in L. multiflorum. |
Palavras-Chave: |
Italian ryegrass. |
Thesagro: |
DNA. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97916/1/atigo-publicado.pdf
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Marc: |
LEADER 01983naa a2200217 a 4500 001 1986699 005 2024-02-05 008 2014 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.2135/cropsci2013.05.0325$2DOI 100 1 $aBUSTAMANTE, F. O. 245 $aDistribution of rDNA in Diploid and Polyploid Lolium multiflorum Lam. and Fragile Sites in 45S rDNA Regions.$h[electronic resource] 260 $c2014 520 $aThe distribution, number, and location of ribosome DNA (rDNA) regions on chromosomes were evaluated, and transcriptional activity was described in genotypes of Italian ryegrass (Lolium multiflorum Lam.), as well as in an offspring resulting from interbreeding. Nucleoli and nucleolar organizer regions were labeled with silver nitrate and fluorescent in situ hybridization (FISH) was performed with 5S and 45S rDNA as probes. Hybridization procedures were performed on slides previously stained with the Ag-NOR method. The 5S rDNA sites were highly conserved, while 45S rDNA sites had wide variability, even showing more than one site on the same chromosome. One of the genotypes had more than expected 45S rDNA sites. Approximately 95% of the cells at metaphases had at least one chromosome break/gap in the 45S rDNA site, resulting in chromosome fragmentation. Thus, 45S rDNA correspond to fragile sites in L. multiflorum. These events can affect genome organization and cause new chromosomal rearrangements which, along with some other factors, might be responsible for microevolutionary changes involved in differentiation and speciation. Not all 45S rDNA sites are transcriptionally active. Variation in both the number and size of nucleoli and mechanisms of nucleolar fusion were observed in L. multiflorum. 650 $aDNA 653 $aItalian ryegrass 700 1 $aROCHA, L. C. 700 1 $aTORRES, G. A. 700 1 $aDAVIDE, L. C. 700 1 $aMITTELMANN, A. 700 1 $aTECHIO, V. H. 773 $tCrop Science$gv. 54, n. 2, p. 617-625 2014.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
04/03/2011 |
Data da última atualização: |
25/05/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
GONDIM, V. I. L.; MORAES, I. V. M. de; LIMA, M. E. de O.; LIMA, J. R.; PINTO, G. A. S. |
Afiliação: |
Victor Igor Lima Gondim, UFC; INGRID VIEIRA MACHADO DE MORAES, CNPAT; Maria Evani de Oliveira Lima, UFC; JANICE RIBEIRO LIMA, CNPAT; GUSTAVO ADOLFO SAAVEDRA PINTO, CNPAT. |
Título: |
Alterações no processamento de suco de caju parcialmente clarificado, tradicionalmente utilizado na província de Nampula/Moçambique, visando maior tempo de conservação. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Resumos... Salvador: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2010. |
Idioma: |
Português |
Palavras-Chave: |
Anacardium occidentale L; Conservantes. |
Thesagro: |
Cajuína; Tratamento Térmico. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/34298/1/RE10105.pdf
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Marc: |
LEADER 00794nam a2200193 a 4500 001 1880050 005 2017-05-25 008 2010 bl uuuu u00u1 u #d 100 1 $aGONDIM, V. I. L. 245 $aAlterações no processamento de suco de caju parcialmente clarificado, tradicionalmente utilizado na província de Nampula/Moçambique, visando maior tempo de conservação. 260 $aIn: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 22., 2010, Salvador. Resumos... Salvador: Sociedade Brasileira de Ciência e Tecnologia de Alimentos$c2010 650 $aCajuína 650 $aTratamento Térmico 653 $aAnacardium occidentale L 653 $aConservantes 700 1 $aMORAES, I. V. M. de 700 1 $aLIMA, M. E. de O. 700 1 $aLIMA, J. R. 700 1 $aPINTO, G. A. S.
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